Role of Nicergoline in Corneal Wound Healing in Diabetic Rats [post]

Amanda Portela, Rafael Moreno, Maria Helena Ribeiro, Fernanda de Andrade, Yale Alves, Mônica Alves, Rodrigo Lira
2020 unpublished
Background: To investigate the effect of nicergoline on the rate of complete corneal ulcer reepithelialization (CCUR) in diabetic rats with diabetic keratopathy.Methods: Forty-eight streptozotocin-induced diabetic rats were randomly divided into two groups. The experimental group (n=24) received nicergoline (10 mg.kg-1.day-1), while the control group (n=24) received a placebo. A corneal epithelial defect was induced using a corneal diamond burr, and defect area was compared at timepoints of 0,
more » ... t timepoints of 0, 12, 24, 48 and 72 hours after the injury using image analysis software. The probability of CCUR within 72 hours was assessed using the Kaplan–Meier survival analysis log-rank test. Results: When compared, 4 of the 24 rats (17%) in the placebo group and 12 of the 24 rats (50%) in the nicergoline group were found to have CCUR within 72 hours (log-rank = 0.027). Cox regression analysis found no effect of the covariates blood glucose (P=0.601) or weight (P=0.322) on the corneal reepithelialization (survival) curve. Conclusions: Nicergoline increased wound healing rates relative to placebo and may therefore be investigated as a treatment option in diabetic keratopathy.
doi:10.21203/rs.3.rs-107020/v1 fatcat:fcrqljsdcfbwxnbih2vj7slxku