Occurrence of pathogenic environmental mycobacteria on surfaces in health institutions

Carlos Henrique Martins
2010 Research and Reports in Tropical Medicine  
The presence of environmental mycobacteria on surfaces in two public health institutions, namely a health center and a hospital in upstate São Paulo (Brazil), was identified by polymerase chain reaction-restriction enzyme analysis (PRA). The possible sources of contamination by these microorganisms were evaluated, contributing to epidemiology studies. Methods: From June 2005 to June 2006, a total of 632 samples were collected from exposed surfaces, such as washbasins, drinking fountains, and
more » ... g fountains, and other accessible sites, and the mycobacteria present in the samples were isolated and cultured. Results: Sixty-five mycobacteria were isolated from the 632 samples; 47 of which were detected in samples from the health center and 18 in samples collected from the hospital. The isolates were identified by DNA restriction patterns obtained by PRA, and potentially pathogenic species were found to be prevalent among the identified mycobacteria. This study shows that the PRA technique can be employed as a fast and easy method for identification of nontuberculous mycobacteria in public areas. Conclusions: The isolation of environmental mycobacteria from the two health institutions demonstrates that these surfaces are reservoirs of potentially pathogenic mycobacteria and indicates the need for continuous maintenance and monitoring. These data will add to the study of the epidemiology of these microorganisms. submit your manuscript | www.dovepress.com Dovepress Dovepress 54 dé Souza et al Research and Reports in Tropical Medicine Publish your work in this journal Submit your manuscript here: http://www.dovepress.com/research-and-reports-in-tropical-medicine-journal Research and Reports in Tropical Medicine is an international, peerreviewed, open access journal publishing original research, case reports, editorials, reviews and commentaries on all areas of tropical medicine, including: Diseases and medicine in tropical regions; Entomology; Epidemiology; Health economics issues; Infectious disease; Laboratory science and new technology in tropical medicine; Parasitology; Public health medicine/health care policy in tropical regions; and Microbiology. The manuscript management system is completely online and includes a very quick and fair peer-review system. Visit http://www.dovepress. com/testimonials.php to read real quotes from published authors. Research and Reports in Tropical Medicine 2010:1 submit your manuscript | www.dovepress.com Dovepress Dovepress 58 dé Souza et al
doi:10.2147/rrtm.s13589 fatcat:qgezb34bbjet7g777wnu6inl3e