Red Blood Cells Actively Contribute to Blood Coagulation and Thrombus Formation [chapter]

Ingolf Bernhardt, Mauro C. Wesseling, Duc Bach Nguyen, Lars Kaestner
2019 Erythrocyte [Working Title]  
The chapter describes the likely molecular mechanisms leading to the aggregation of human red blood cells (RBCs) under conditions of physiological coagulation when prostaglandin E 2 (PGE 2 ) or lysophosphatidic acid (LPA) is released from activated platelets and under pathophysiological conditions, in particular thrombi formation in sickle cell disease when patients are in a vaso-occlusive crisis. In both scenarios cation channels are activated. This leads to an increase of the free
more » ... he free intracellular Ca 2+ concentration resulting in an activation of the lipid scramblase, which in turn mediates a movement of phosphatidylserine (PS) from the inner to the outer membrane leaflet. In addition, the increased Ca 2+ concentration leads to the activation of the Gardos channel. Experiments suggesting this mechanism have been performed with fluorescence microscopy, flow cytometry as well as single-cell force spectroscopy. The Ca 2+ -triggered RBC aggregation force has been identified to be close to 100 pN, a value large enough to play a significant role during thrombus formation or in pathological situations.
doi:10.5772/intechopen.86152 fatcat:vrv2n2csbfdhrnscogkzfzq7gu