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35 European Journal of Biotechnology and Bioscience
The mammalian ovaries are valuable source of oocytes for reproductive biotechnology. Oocytes attain developmental competence in vivo being in follicular fluid so using it as maturation medium could be an option. Swine, bovine, horse, ovine and even murine IVM studies using follicular fluid gave favorable effects. Bubaline IVM experiment in CRD made use of 271 oocytes from buffalo ovaries. The oocytes were subjected to five maturation media: pure TCM 199 (T 1); 50% TCM 199 + 50% BuFF from 2-5 mmfatcat:r72ggrdvtbelbapyohhjhw2ccq