Extraction of glabridin with heat reflux extraction by response surface analysis

Li-ying DING, Hong-yan LI
2013 Global Journal of Integrated Chinese Medicine and Western Medicine  
Objective: To study the extraction process of glabridin from Glycyrrhiza glabra. Methods: The solid-liquid ratio, extraction time and extraction temperature are the three main factors in the experiment, and the extract solvent is ethyl acetate. Establish the response surface quadratic regression equation of glabridin extraction rate and purity. Results:The results indicate that the optimum conditions for glabridin are as follows: solvent is ethyl acetate, extraction time is 90 min, the ratio of
more » ... 0 min, the ratio of solvent to solid is 35 ∶1 (ml/g), and extraction temperature is 44.7 ℃, purity and the maximum yield of Extraction of glabridin are 6.96% and 0.241%, The validation test shows that the experimental values of the model are 0.235% and 6.05% . Conclusion: The model equation can predict the experimental results, it can be used as reference in industrial production.
doi:10.14725/gjicmwm.v1n1a58 fatcat:sneyosd5a5hcrpupnxqgo446u4