Fluorescence Enhancement Effect of TiO2 Nanoparticles and Application for Photodynamic Diagnosis

Koki Kanehira, Yukiko Yano, Hisashi Hasumi, Hideo Fukuhara, Keiji Inoue, Kazuhiro Hanazaki, Masahiro Yao
2019 International Journal of Molecular Sciences  
Photodynamic diagnosis (PDD) can improve diagnostic accuracy by using PDD agents such as 5-aminolevulinic acid (ALA). However, the weakness and photobleaching of fluorescence of PDD agents may lead to insufficient fluorescence visibility for the detection of cancer during resection operations. We focused on the "fluorescence enhancement effect" resulting from the addition of polyethylene glycol-modified titanium dioxide nanoparticles (TiO2-PEG NPs) to address these problems. The results showed
more » ... hat the combined administration of TiO2-PEG NPs and ALA could enhance and prolong fluorescence in bladder cancer cells, similar to in the mixture alone. It was suggested that the fluorescence enhancement was related to the accumulation of TiO2-PEG NPs in cells via endocytosis, causing the light scattering and enhancement of fluorescence. This fluorescence enhancement effect could be applicable for PDD.
doi:10.3390/ijms20153698 fatcat:mi5ugegxnvbdzichdxopsjzymq