HIV-1 Tat-mediated protein transduction of human brain creatine kinase into PC12 cells

Min-Seop Jeong, Dae-Won Kim, Min-Jung Lee, Yeom-Pyo Lee, So-Young Kim, Sun-Hwa Lee, Sang-Ho Jang, Kil-Soo Lee, Jin-Seu Park, Tae-Cheon Kang, Sung-Woo Cho, Oh-Shin Kwon (+2 others)
2008 BMB Reports  
Epilepsy is characterized by the presence of spontaneous episodes of abnormal neuronal discharges and its pathogenic mechanisms remain poorly understood. Recently, we found that the expression of creatine kinase (CK) was markedly decreased in an epilepsy animal model using proteomic analysis. Tat -CK provides a strategy for the therapeutic delivery of proteins in various human diseases including the delivery of CK for potential epilepsy treatment. [BMB reports 2008; 41(7): 537-541] A human CK
more » ... 7-541] A human CK gene was fused with a HIV-1 Tat peptide to generate an in-frame Tat-CK fusion protein. The purified Tat-CK fusion protein was efficiently transduced into PC12 cells in a time-and dose-dependent manner when added exogenously to culture media. Once inside the cells, the transduced Tat-CK fusion protein was stable for 48 h. Moreover, the Tat-CK fusion protein markedly increased endogenous CK activity levels within the cells. These results suggest that
doi:10.5483/bmbrep.2008.41.7.537 pmid:18682038 fatcat:ad4mxwzk2jgprdfdk2odfddor4