Decreased MEFV gene expression in rheumatoid arthritis patients

E.O. Etem, S.S. Koca, D. Erol, S. Yolbas, E. Oz, H. Elyas, A. Isık
2015 Genetics and Molecular Research  
Rheumatoid arthritis (RA) is a major cause of adult chronic inflammatory arthritis and an autoimmune disease of unknown etiology in which the inflammatory pathology involves T cell activation. Genetic mutations in the Mediterranean fever (MEFV) gene, encoding pyrin, influence the severity of RA, but the underlying mechanisms are not completely understood. In this study, we investigated whether the full-length MEFV gene (MEFV-fl) and the exon 2-deleted splice isoform (MEFV-d2) expression are
more » ... expression are associated with or responsible for the clinical conditions of RA. This study include 47 patients with RA and 47 age-and gender-matched healthy controls. Quantitative realtime polymerase chain reaction analysis was performed to examine transcriptional changes in MEFV gene expression from peripheral blood samples. Reverse transcription-polymerase chain reaction of peripheral blood cells revealed the downregulation of MEFV-fl mRNA in non-treated patients compared with healthy controls and treated patients. MEFV-d2 expression was not different between groups. This
doi:10.4238/2015.february.6.3 pmid:25730039 fatcat:lxq6eyqfmjgnpc7mueochxtpke