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Post-ischemic reperfusion leads to apoptosis-linked loss of myocytes in cultured cells and in vivo. We tested the hypothesis that apoptosis develops without reperfusion in Langendorffperfused hearts exposed to either low-flow ischemia (LFI) or hypoxia (H). Rat hearts were perfused with aminoacid-enriched Krebs-Henseleit buffer and exposed for 6 h to LFI (flow=2 ml/min, PO 2 =500±50mmHg, mean±SD), H (10ml/min, 120±15mmHg), or control conditions (C, 10ml/min, 500±50mmHg). At selected times,doi:10.1159/000047825 pmid:11914547 fatcat:glackebadnbp3m7waymskr7wee