Cardiovascular diseases are described under Hridroga in Ayurvedic classics. Trinetra Rasa is a herbo-mineral formulation explained in the context of Hridroga. The present study was aimed to evaluate Cardio protective activity of Trinetra Rasa against Isoprenaline induced cardiac damage in rats and attempt to understand mechanism of its therapeutic effect with respect to biochemical markers, ECG and Histopathological changes. Albino rats of Wistar strains of either sex between 150 to 250 g,

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... mly divided into 6 groups, 8 rats in each group. Group 1-Control group given tap water 10 ml/kg, Group 2-Isoprenaline control group -100 mg/kg, Group 3-Standard group given Proprenalol-10 mg/kg, Group 4-Honey (Vehicle control/Anupana)-10 ml/kg (Test 1), Group 5-Trinetra Rasa in therapeutic dose along with Honey-750 mg/kg (Test 2) and Group 6-Trinetra Rasa in 2 times the therapeutic dose along with Honey-1500 mg/kg (Test 3) were administered according to the body weight of animals by oral route to respective groups at morning hours and continued for 20 days. On 19 th day, ECG was recorded to assess Myocardial infarction and 1 st dose of Isoprenaline injection (s/c) was given, on 20 th day after 24 hours of 1 st dose, 2 nd dose of Isoprenaline injection was given (s/c) and ECG was recorded. Biochemical and histopathological assessment was done. In biochemical parameters better effect was observed with Test 2 group but histopathological profile showed good effect at Test 3 group higher dose level. The study provided evidence for presence cardio protective activity of Trinetra Rasa against Isoprenaline induced cardiac damage in rats.