Plant Cell Contact-Dependent Virulence Regulation of hrp Genes in Pseudomonas syringae pv. tabaci 11528
Pseudomonas syringae pv. tabaci 에서 식물세포접촉에 의한 병원성 유전자의 조절

Jun-Seung Lee, Ji-Young Cha, Hyung-Suk Baik
2011 Journal of Life Science  
The hrp gene cluster in the plant pathogen Pseudomonas syringae is a key determinant of pathogenicity. Recent studies have demonstrated that specific host cell induction of the Ralstonia solanacearum hrp gene cluster is controlled by the PrhA (plant regulator of hrp) receptor. To characterize the role that P. syringae PrhA plays in the virulence of plant cells, a prhA homolog was isolated from P. syringae pv. tabaci and a ΔprhA mutant was constructed by allelic exchange. The ΔprhA mutant had
more » ... ΔprhA mutant had reduced virulence in the host plant, and co-culture of P. syringae pv. tabaci and plant cell suspensions induced a much higher level of hrpA gene transcription than culture in hrp-inducing minimal medium. These results indicate that PrhA of P. syringae is a putative pathogen-plant cell contact sensor, therefore, we used a hrpA-gfp reporter fusion to monitor the in situ expression of PrhA. The results of this study demonstrated that PrhA induces hrp gene expression in P. syringae pv. tabaci in the presence of plant cells.
doi:10.5352/jls.2011.21.2.227 fatcat:abp56i5sjzhqjjjtsrcssnqy6i