Use of theLitomosoides sigmodontis- Mouse model in development of anOnchocercavaccine. II -L. sigmodontisin the balb/c mouse: vaccination experiments; preliminary immunological studies

P. Maréchal, G. Petit, M. Diagne, D.W. Taylor, O. Bain
1994 Parasite  
Early experiments demonstrated genetic restriction in the pattern of infection with L. sigmodontis in various inbred strains of mice (Petit et al., 1992) . BALB/c mice were the most susceptible and this strain has been used for vaccina tion experiments. FATE OF L SIGMODONTIS IN BALB/C MICE ; VACCINATION pwo months after inoculation with normal 25 L3, all mice inoculated harboured adult filariae with a mean of 4.6 worms (range from 1.5 to 10.6 based on 9 experiments with 7 to 15 mice per
more » ... 15 mice per experiment), and 45 % of mice presented with a microfilaremia (range between 25 and 86 % for indi vidual experiment). Similar results were obtained when mice were either 1-or 2-month old at time of inoculation. Different protocols of vaccination with irradiated infective larvae were investigated. Best results were obtained when mice were inoculated at weekly intervals with three doses of 25 L3 irradiated with 60 Krads : this regimen resulted in a 83 % reduction in worm burden.
doi:10.1051/parasite/199401s1031 fatcat:lpdkkubtpjhktj5arnkomiwvre