Investigations of the fragmentation behavior of 11 isoflavones with ESI-IT-TOF-MSn

Yazhou Zhang, Hubiao Chen
2014 Journal of Chinese Pharmaceutical Sciences  
The fragmentation behavior of isoflavones was studied using electrospray ionizationion traptime of flight mass spectrometry (ESIITTOFMS n ). It was found that the isoflavone glycoside bond was easily broken. The fragmentation occurred mostly on the Cring, and the fragment ions of A 1,3+ produced by the RDA cracking will predict the hydroxylation replacement on Aring or Bring. In addition, four carbonyl groups on the Cring were fragmented through neutral loss of 28 (CO). A and Brings primarily
more » ... Brings primarily lose substituents which including a neutral losses of 32 (CH 3 OH), 16 (CH 4 ), or 16 (O), and 18 (H 2 O). Aring in the presence of adjacent hydroxylation, also easily made to be a neutral losses of 28 (CO) or 18 (H 2 O). It is likewise common to see methoxy replaced with a neutral losses of 16 (CH 4 ) or 32 (CH 3 OH) in Bring, also the hydroxylation on benzene ring can occasionally results with the neutral loss of 28 (CO).
doi:10.5246/jcps.2014.09.081 fatcat:62iwkfqtvjbdtoqur5u6xlsupa