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Assessment of DNA Damage and Apoptosis in the Brain of Freshwater Fish Oreochromis mossambicus Exposed to Tannery Effluent Wastewater

N. Ganesan, K. Samyappan, R. Saravanan
2021 Applied Ecology and Environmental Sciences  

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The present study was designed to evaluate the effect of tannery effluent on the fingerlings of freshwater fish Oreochromis mossambicus. The fishes were maintained at 10% concentration of tannery effluent for a period of 30 days. Apoptosis was studied by activity of caspases. Caspase 3 and 9 activity showed significant alteration. DNA seperation by agarose gel electrophoresis shows difference in intensity of genomic DNA between 750-1000 bp in fishes exposed to tannery effluent. DNA damage was
more » ... so confirmed by Comet assay which showed an increase in DNA damage and tail migration was indicated by increase in percentage of mean DNA comet tail length formation.
doi:10.12691/aees-9-5-8 fatcat:7t74ko7usjh6zlfyyauvnngjs4
Citation

N. Ganesan, K. Samyappan, R. Saravanan. "Assessment of DNA Damage and Apoptosis in the Brain of Freshwater Fish Oreochromis mossambicus Exposed to Tannery Effluent Wastewater." Applied Ecology and Environmental Sciences 9.5 (2021) 575-579