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The flow cytometry analysis of human peripheral blood monocytes separated by a two step density gradient centrifugation is reported. The expression of mRNA level of adenosine receptor (AdoR) subtypes (A1, A2A, A2B and A3) and interleukin 6 (IL-6), interleukin 8 (IL-8) and vascular endothelial growth factor (VEGF) in monocytes were determined using real-time PCR. We found considerable variation across individuals in mRNA expression levels of paracrine factors after the stimulation of adenosinedoi:10.20538/1682-0363-2011-3-54-61 doaj:a2772ef9bab5410da37349c1c2cb575d fatcat:grxwwqc4x5db5p3ietwybxmvva