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The objective of this study is to synthesize ZnO nano particles in form thin film on glass substrate and study antibacterial active of such films. ZnO thin films were prepared by spray pyrolysis technique using zinc acetate and isopropyl alcohol as precursor in the temperature range 375̊C to 425̊C. The optical properties of the film were studied on UV/VIS/NIR spectrophotometer. The energy gap of the film is evaluated and it is found to be 3.31 eV. The polycrystalline nature of the ZnO film wasdoi:10.46501/ijmtst0702003 fatcat:bpfzcpmvpzhkbmujsiyftnzjhq