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Methods Preparation of Animals Male golden hamsters (105 ± 1.49 g, n = 73) were anesthetized with sodium pentobarbital (Nembutal, Abbott; 70 mg/kg administered intraperitoneally). Tracheal and femoral venous cannulae were inserted and the animals received an infusion (0.44 ml/hr) of Nembutal diluted with saline (9.1 mg/ml) to maintain the appropriate level of anesthesia and to replace fluids lost due to surgery, renal function, and respiratory evaporation. Fluid infusion at this rate maintaineddoi:10.1161/01.res.53.4.515 pmid:6627610 fatcat:gehih3smmbdijavoothistbq4u