The oxygen sensitivity of hamster cheek pouch arterioles. In vitro and in situ studies

W F Jackson, B R Duling
1983 Circulation Research  
Methods Preparation of Animals Male golden hamsters (105 ± 1.49 g, n = 73) were anesthetized with sodium pentobarbital (Nembutal, Abbott; 70 mg/kg administered intraperitoneally). Tracheal and femoral venous cannulae were inserted and the animals received an infusion (0.44 ml/hr) of Nembutal diluted with saline (9.1 mg/ml) to maintain the appropriate level of anesthesia and to replace fluids lost due to surgery, renal function, and respiratory evaporation. Fluid infusion at this rate maintained
more » ... his rate maintained animal hematocrits constant and mean arterial pressure constant at 100 mm Hg for up to 6 hours. Deep esophageal temperature was maintained at 37°C by conductive hearing.
doi:10.1161/01.res.53.4.515 pmid:6627610 fatcat:gehih3smmbdijavoothistbq4u