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A SERUM-DEPRIVED HUMAN EMBRYO PANCREATIC ISLET CELL CULTURE SYSTEM: A CULTURE METHOD SUITABLE FOR DOUBLE-LABEL ANTIBODY TECHNIQUE TO DETECT VIRUS INFECTION OF BETA CELLS
Japanese Journal of Medical Science & Biology
By the use of serum-free Eagle's MEM instead of serum-supplemented culture medium in human embryo islet cell culture, it was possible to increase the proportion of beta cells identified by the indirect fluorescent antibody (IFA) technique. With the combination of the serum-deprived culture system and the doublelabel antibody technique, it was possible to show that the human embryo beta cells in culture were susceptible to infection with human papovavirus BK (BKV). Furthermore, this combinationdoi:10.7883/yoken1952.36.85 fatcat:uzanbbhulfcmlfj5cfg4kpu3ke