Bimolecular Interaction of Insulin-Like Growth Factor (IGF) Binding Protein-2 with αvβ3 Negatively Modulates IGF-I-Mediated Migration and Tumor Growth1

Joseph J. Pereira, Tim Meyer, Susan E. Docherty, Hugh H. Reid, John Marshall, Erik W. Thompson, Jamie Rossjohn, John T. Price
2004 Cancer Research  
Both the integrin and insulin-like growth factor binding protein (IGFBP) families independently play important roles in modulating tumor cell growth and progression. We present evidence for a specific cell surface localization and a bimolecular interaction between the ␣v␤3 integrin and IGFBP-2. The interaction, which could be specifically perturbed using vitronectin and ␣v␤3 blocking antibodies, was shown to modulate IGF-mediated cellular migration responses. Moreover, this interaction was
more » ... ved in vivo and correlated with reduced tumor size of the human breast cancer cells, MCF-7␤3, which overexpressed the ␣v␤3 integrin. Collectively, these results indicate that ␣v␤3 and IGFBP-2 act cooperatively in a negative regulatory manner to reduce tumor growth and the migratory potential of breast cancer cells.
doi:10.1158/0008-5472.can-03-3056 pmid:14871828 fatcat:6ub3nlh7x5fohernqpmgwubit4