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Determination of annonacyn grade, a main insecticide compound has been done in the ethanolic and ethyl acetate extracts produced by soxhletation, and ethyl acetate extract produced by fractionation of ethanolic extract to find out the most active extract to be used as raw material of pesticide formulae. Analysis method used the reverse phase high performance liquid chromatography with column of Novapack ODS C-18 (waters; 3.9 x 150 nm), mobile phase was the mixture of acetonitrile-water. Thedoi:10.22146/ijc.21792 fatcat:c5nb53hyujgrlp2d7kg4wqfofi