Polymorphisms in genes CTSB, CTSD, CAPN2, KLK1 and TGFB1 not associated with susceptibility to atypical or classical ovine scrapie

D. K. Caspari, A. Balkema-Buschman, H. R. Brandt, M. H. Groschup, G. Erhardt, G. Lühken
2010 Archives Animal Breeding  
In the present study polymorphisms in the genes cathepsin B (<i>CTSB</i>), cathepsin D (<i>CTSD</i>), calpain, large polypeptide L2 (<i>CAPN2</i>), kallikrein 1 (<i>KLK1</i>) and transforming growth factor β1 (<i>TGFB1</i>) were investigated for association with scrapie susceptibility in sheep. Therefore single nucleotide polymorphisms in the respective genes were identified and examined for a potential impact on the gene function with different computer programs. Samples of 72 atypical and 104
more » ... 72 atypical and 104 classical scrapie cases as well as of 443 clinically healthy flock mates were genotyped by PCR-based screening methods. Neither allele frequencies nor genotype frequencies showed significant differences between scrapie positive sheep and control animals in any of the investigated genes.
doi:10.5194/aab-53-457-2010 fatcat:oc7yadz3qbeijit7jap2qqnzgu