Keragaman Baru pada Daerah Ujung Gen Hormon Pertumbuhan Sapi Pesisir Ternak Lokal Sumatera Barat

Yurnalis Yurnalis, Arnim Arnim, Sarbaini Sarbaini, Jamsari Jamsari
2017 Jurnal Peternakan Indonesia  
ABSTRAK Dari 210 sampel sapi pesisisir umur 1,5 tahun, dipilih 60 sample berdasarkan berat badan yaitu 30 sampel dengan berat tinggi (125±9,02 kg) dan 30 sampel dengan berat badan rendah (65±6,09 kg). Sampel darah 60 ekor sapi diisolasi dan produk PCR dari fragmen gen GH (455 bp) disekuensing forward dan reserve. Dua dilesi ditemukan pada posisi 2731 dan 2732 dengan fekuensi allel 0.06, dan 0.19. Delapan mutasi ditemukan pada posisi A dengan frekuensi allel berturut-turut 0,66; 0,97; 1,00;
more » ... 6; 0,97; 1,00; 0,54; 0,37; 0,62; 0,77 dan 1,00. Hasil penelitian ini menunjukkan gen GH pada sapi pesisir mempunyai keragaman yang tinggi, sehingga perlu dilakukan penelitian lanjut untuk mengetahui apakah keragaman ini berhubungan dengaan sifat-sifat produksi. ABSTRACT From a total of 210 of 1,5-year-old Pesisir cattle, 60 were selected on the basis of weight, those were 30 with highest weight (125±9,02 kg) and 30 with lower weight (65±6,09 kg). DNA isolated from 60 blood sample and PCR product of GH fragment (455 bp) were analyzed by direct sequencing. Two deletions were detected in position 2731 and 2732 with frequency allele were 0.06, and 0.19. Eight mutation were detected in position 2537, A with frequency allele were 0,66; 0,97; 1,00; 0,54; 0,37; 0,62; 0,77 and 1,00 respectively. These data provide evidence that GH gene is good polymorphic source and can be used for association with performance and investigate whether these polymorphic responsible for quantitative variation in growth.
doi:10.25077/jpi.19.3.103-109.2017 fatcat:uqos4hvcdnbkbhyskj65l673la