Determinación por medio de marcadores moleculares SSCP y RAPD de la diversidad genética en la especie Taenia solium en Colombia

MARCELA FERNÁNDEZ, AMALIA MUÑOZ, MAURICIO CORREDOR
2006 Parasitología latinoamericana  
SSCP and RAPD techniques were performed in order to detect the genetic variability of Taenia solium cestode, using both mitochondrial and nuclear DNA fragments. The DNA was extracted from eight different cysts isolated of pigs originated from three distant Colombian provinces: Antioquia, Nariño and Sucre. Gene fragments corresponding to NADH dehydrogenase 1 (ND1) and cytochrome oxidase c subunit I (COI) were amplified by PCR using total DNA from individual cysts and later run on non denaturing
more » ... on non denaturing SSCP acrylamide gels. Three different patterns were obtained by SSCP for both genes indicating that ND1 and COI mitochondrial genes are polymorphic in Colombian T. solium species. COI patterns were more polymorphic, related to the geographical origin. Furthermore, RAPD decameric primers showed a nuclear polymorphic DNA, that corroborates the genetic diversity between this isolates.
doi:10.4067/s0717-77122006000200001 fatcat:ivoiai4azreo5fjsm5f46zawwq