Screening of Specific Changes in mRNAS in Thyroid Tumors by Sequence Specific Differential Display. Decreased Expression of c-fos mRNA in Papillary Carcinoma

GANG LIU, TORU TAKANO, FUMIO MATSUZUKA, TAKUYA HIGASHIYAMA, KANJI KUMA, NOBUYUKI AMINO
1999 Endocrine journal  
To examine the specific changes in gene expression in thyroid carcinomas, we performed sequence specific-differential display (SS-DD) analysis by using a specific degenerate primer for the superfamily of the small G protein. After subcloning and sequencing analysis, one of the genes that showed decreased expression in thyroid papillary carcinomas was revealed to be the proto-oncogene c fos. Expression of c fos mRNA in benign and malignant tissues was examined by reverse transcription-polymerase
more » ... cription-polymerase chain reaction (RT-PCR) and Northern blotting. The expression of c fos was detected in all twelve normal thyroid tissues, whereas it decreased in thirteen of fifteen papillary carcinomas. These results indicate that the increased expression of c fos mRNA is not necessarily to be an oncogenic feature of thyroid tumors. Further, its constant expression in normal thyroid tissues may play a role in the maintenance of thyroid function.
doi:10.1507/endocrj.46.459 pmid:10504000 fatcat:ccgf3nftcneh5blc6f4t6wly34