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In vitro microtuber induction potential of a popular potato (Solanum tuberosum L.) variety Diamant was examined using different supplements of growth regulators. Results showed that MS supplemented with 4.0 mg/l BAP, 1.0 mg/l IAA and 1.0 mg/l GA3 produced the best response towards microtuber induction from in vitro raised stem cuttings. Further, these in vitro raised microtubers were used to establish a highly efficient shoot regeneration system. Highest number of regenerated shoots weredoi:10.3329/ptcb.v29i1.41979 fatcat:3hjqocrhczc73cxwgrcgwtt7m4