Integrate analysis and identification for different expression genes in chondrogenesis [post]

2020 unpublished
The intricate mechanisms of articular chondrogenesis are largely unknown. Gradually, with the help of high-throughput platforms, microarrays have become an important and useful method to testify hub genes in desease. Today, advanced bioinformatic analysis of available microarray data can provide more reliable and accurate screening results by duplicating related data sets. Results : Microarray datasets GSE9451 and GSE104113 were downloaded from Gene Expression Omnibus (GEO) database. The
more » ... atabase. The differentially expressed genes (DEGs) were performed, and function enrichment analyses were demonstrated. The proteinprotein interaction network (PPI) was constructed and the module analysis was performed by using STRING and Cytoscape. Quantitative PCR was used to confirm the results of bioinformatics analysis. Conclusion: Compared to individual studies, this study can provide extra reliable and accurate screening results by duplicating relevant records. Additional molecular experiments are required to confirm the discovery of candidate genes identified by chondrogenesis. S100A4 is predicted to integrate with miR-325-3p to promote osteogenesis. Background Chondrogenesis happens in fracture healing and skeleton development. The intricate mechanisms of articular chondrogenesis are largely unknown. It starts with proliferation, condensation and recruitment of mesenchymal progenitor cells which finally cause the form of a precartilaginous primordium [1]. Additionally, this process is programmed in genomic sequences [2-4].
doi:10.21203/rs.2.20142/v1 fatcat:5xpf3edezzhhndaszxlzb3us44