Studying gene function in Caenorhabditis elegans using RNA-mediated interference

E. M. Maine
2008 Briefings in Functional Genomics & Proteomics  
The RNA interference (RNAi) method for targeted gene silencing is widely used in Caenorhabditis elegans for largescale functional genomic studies, analysis of limited gene sets and detailed analysis of individual gene function. The application of RNAi has identified genes that participate in various aspects of development, physiology and cell biology. In addition, RNAi has been used to identify interacting genes and to study functionally redundant genes. This review discusses the various
more » ... tions of RNAi in C. elegans, focusing particularly on the analysis of developmental processes. Key Points RNAi-mediated gene silencing is a versatile method for studying gene function. RNAi-based gene interaction screens are identifying components of known biochemical and cellular pathways. RNAi-based screens are identifying proteins that function in defined developmental and cellular processes. RNAi-mediated gene silencing is providing information for the assembly of gene interaction networks.
doi:10.1093/bfgp/eln019 pmid:18443013 fatcat:2m6wv53p65ga7pu5m6qemm73yy