Self-regulation of neutrophils during phagocytosis is modified after severe tissue injury

Gábor Pap, József Furész, János Fennt, Gábor Kovács, László Nagy, János Hamar
2006 International Journal of Molecular Medicine  
Neutrophil (PMNL) function is influenced by factors released by other immune cells during the course of the immune response. We investigated the effect of neutrophil cell density and the effect of supernatant of the phagocytosis assay on the phagocytosis activity of PMNLs. The measurements were carried out with naive (control) PMNLs of healthy donors and with PMNLs obtained from patients with severe tissue injury. Phagocytosis index (FI) of PMNLs was determined at cell densities of 7.5x10 5 /ml
more » ... and 15x10 5 /ml. E. coli phagocytosis of heparinized whole blood from healthy donors and patients with severe tissue injury was measured and evaluated at three different cell densities (normal, half, and double densities) by flow cytometry.
doi:10.3892/ijmm.17.4.649 fatcat:e2bfwiqjozcy5oleif4lb7x2ri