Persicaria thunbergii Extract as a Physiologically Active Cosmetic Ingredient
Asian Journal of Beauty and Cosmetology
Purpose: The purpose of this study was to investigate the potential of Persicaria thunbergii (P. thunbergii) extracts as physiologically active cosmetic ingredients. Methods: To elucidate the anti-oxidative, anti-inflammatory, and anti-microbial properties of P. thunbergii , its ethanol and hot-water extracts were examined for 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity, nitric oxide (NO) production, protective effects against oxidative stress in HaCaT cells,
... lls, antiinflammatory activity, anti-microbial activity, and inhibition of β-hexosaminidase expression, an allergy factor. Results: The anti-oxidative activity of the two P. thunbergii extracts was compared, and the anti-oxidative activity of the ethanol extract was found to be superior to that of the hot-water extract. The polyphenol contents of P. thunbergii ethanol and hot-water extracts were 132.00 and 89.46 μg/ mL, respectively. And total flavonoid contents of P. thunbergii ethanol and hot-water extracts were 115.27, 84.94 μg/mL, respectively. The DPPH radical scavenging activity was found to be 79.80% for the ethanol extract and 70.33% for the hot-water extract at a concentration of 500 μg/mL. No significant cytotoxicity was observed in HaCaT, RAW 264.7 and RBL-2H3 cells. The protective effect of the extracts on HaCaT cell against oxidative stress induced by hydrogen peroxide (H 2 O 2 ) was confirmed by 23% for ethanol extract and 18% for hot water extract. The anti-inflammatory activity of the extracts was examined in RAW 264.7 cells, and nitric oxide (NO) production was suppressed in a concentration-dependent manner. Both ethanol and hotwater extracts also inhibited the degranulation of immune cells in a concentrationdependent manner, as assessed by the secretion of β-hexosaminidase. In addition, the concentration dependent anti-microbial activities of the extracts were demonstrated in several bacterial strains, such as those of Staphylococcus aureus (S. aureus), Staphylococcus epidermidis (S. epidermidis), and Propionibacterium acnes (P. acnes). Conclusion: Based on the findings from this study, P. thunbergii extracts could be used as functional cosmetic ingredients that possess anti-oxidative, antiinflammatory, and anti-microbial properties. In order to determine their applicability, further research should be systematically conducted to verify their efficacy through clinical studies in animals and humans. EB. Inhibitory effect of extracts from Rhododendron Brachycarpum and Abies Koreana E.H. Wilson on degranulation and cytokine expression in RBL-2H3 cells.