and Objective: This research was conducted to determine the effect of sulfur and cattle manure on yield and amino acids of two Sesame (Sesamum indicum L.) varieties. Materials and Methods: The experiment was done by a factorial experiment in randomized block design with 3 replications in 2016 and 2017 at Kerman Agricultural and Natural Resources Research Center. The treatments consisted of powdered sulfur at four levels (0, 2, 4 and 8 ton.ha-1), Cow manure at two levels (0 and 20 ton.ha-1) and

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... iroft-13 and Jiroft local varieties. Results: The results showed that the number of capsules per plant in the second year was higher than the first year and the number of seeds per capsule in the second year was lower. Also, the leaf phosphorus content in the second year was higher than the first year. The number of branches, number of capsules per plant, 1000-grain weight, grain yield and Methionine in 20 ton.ha-1treatment of cow manure was higher than the control. The effect of sulfur treatment on yield, yield components, seed oil, Leaf phosphorus, Methionine and Cysteine were significant and according to the significance of the interaction between cow manure and sulfur, the highest grain yield (1353.28 Kg.ha-1) was obtained from of 20 Ton.ha-1 treatment of cow manure and 8 ton.ha-1of sulfur. The Jiroft-13 variety had higher oil seed, grain and biological yield than Jiroft local variety.Conclusion: According to the experiment conditions, more desirable results can be obtained using 20 ton.ha-1 manure, with lower rates of sulfur.