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A construct (AT1R-NF) containing a "Flag" sequence added to the Nterminus of the rat AT 1 receptor was stably expressed in Chinese hamster ovary cells and quantified in the cell membrane by confocal microscopy after reaction with a fluorescein-labeled anti-Flag monoclonal antibody. Angiotensin II bound to AT1R-NF and induced endocytosis with a half-time of 2 min. After 60-90 min, fluorescence accumulated around the cell nucleus, suggesting migration of the ligand-receptor complex to the nucleardoi:10.1590/s0100-879x2001000900011 pmid:11514842 fatcat:4gjizkwmerfwngrwlvmrimfzfe