The study conducted to isolate Pseudomonas spp. from soil and water sources. The identification of Pseudomonas spp. performed by staining and biochemical testing. The biochemical test suggests isolated strains from garden soil, petroleum-contaminated soil, edible oil-contaminated soil, metal contaminated soil, and contaminated seawater, possess similar characteristics like Pseudomonas fluorescence, Pseudomonas aeruginosa, Pseudomonas alkaligens, Pseudomonas mendocina, Pseudomonas putida

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... vely. Molecular fingerprinting is necessary to confirm their presence. Antimicrobial susceptibility of Pseudomonas spp. against different antibiotics was performed by Kirby-Baur Method. In the susceptibility assay, five different antibiotics tested. Pseudomonas isolates showed the highest susceptibility against Gentamicin and polymyxin B, as well as it shows complete resistance to amikacin, cefotaxime, and vancomycin.