Transcellular transport of lipoprotein through arterial endothelial cells in monolayer culture

Ryoichi Hashida, Chie Anamizu, Junji Kimura, Shoji Ohkuma, Yoji Yoshida, Tatsuya Takano
1986 Cell Structure and Function  
To study the mechanism of lipoprotein transport through arterial endothelial cells, porcine endothelial cells were cultured on gelated type I collagen supported by a dacron sheet, and the transport of low density lipoprotein (LDL) labeled with rhodamine B isothiocyanate (RB-LDL) through the cells was measured. Light and scanning electron microscopy showed that the cells on the gel were confluent. There was little RB-LDL transport through the endothelial monolayer at 0°C. RB-LDL transport
more » ... DL transport through the monolayer at 37°C was dose-dependent saturable at 0.4 mg protein/ml. The transport was energy-dependent, since its rate was affected by temperature and was inhibited by a combination of 2-deoxyglucose (50 mM) and NaN3 (10 mM). RB-LDL was shown not to be degraded during transport.
doi:10.1247/csf.11.31 fatcat:mkuuzhnsubdzrdiwx65v6kcnba