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New approach for optical imaging, structural study and cell cultivation based on the effect of the enhancement of optical signals from biomolecules and biological cells near nanostructured rough gold surface is proposed. The surface enhanced IR absorption (SEIRA) spectroscopy and confocal microscopy experiments were made using the culture of SPEV (porcine embryonic kidney epithelium transplantable line) and fibroblast cells, cultivated and/or adsorbed on the gold substrate. The SEIRA spectradoi:10.15407/spqeo20.02.159 fatcat:nuqtxzvzubed5l64smmuuzgmce