Safety Evaluation of Chinese Medicine Injections with a Cell Imaging-Based Multiparametric Assay Revealed a Critical Involvement of Mitochondrial Function in Hepatotoxicity

Meng Wang, Chen-Xiang Liu, Ran-Ran Dong, Shuang He, Ting-Ting Liu, Tie-Chan Zhao, Zhi-Long Wang, Xi-Ya Shen, Bo-Li Zhang, Xiu-Mei Gao, Yan Zhu
2015 Evidence-Based Complementary and Alternative Medicine  
The safety of herbal medicine products has been a widespread concern due to their complex chemical nature and lack of proper evaluation methods. We have adapted a sensitive and reproducible multiparametric cell-based high-content analysis assay to evaluate the hepatic-safety of four Chinese medicine injections and validated it with classical animal-based toxicity assays. Our results suggested that the reported hepatotoxicity by one of the drugs, Fufangkushen injection, could be attributed at
more » ... st in part to the interference of mitochondrial function in human HepG2 cells by some of its constituents. This method should be useful for both preclinical screen in a drug discovery program and postclinical evaluation of herbal medicine preparations.
doi:10.1155/2015/379586 pmid:25792997 pmcid:PMC4352439 fatcat:wskmmltx75dftdqyucnj4x3xja