The experiment to study an effect of bentonite addition in the pelletizing process of hatchery wastes on Coliform and Salmonella number of pellet products after storage in different times. The experiment was conducted at the Feed Technology Laboratory, Faculty of Animal and Agriculture Sciences, Diponegoro University. Bentonite has a property of adsorbing substances and has a larger particle pore size than bacteria, thus enabling the occurrence of bacterial adsorption into the bentonite

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... e and inhibiting the growth of bacteria. Therefore, bentonite is expected to elongate the life time of pellet products during storage. The experiment was conducted by Completely Randomized Design (CRD) with factorial pattern 2×3. The first factor was the level of bentonite; 0 and 3%, and the second factor was time of storage; 4, 8 and 12 weeks with three replications of each treatment combination. The results showed that the addition of 0 and 3% bentonite had no significant effect (P>0.05) on the number of Coliform in the hatchery wastes pellets after storage. Moreover, Salmonella in the hatchery waste pellets was negative. It could be concluded that pelleting process was able to suppress the number of Coliform and Salmonella of hatchery waste pellets until a critical limit of safety number, but the addition of bentonite was not effective on inhibiting the growth of pathogenic bacteria during storage.