Influence of initial L-asparagine and glycerol concentrations on the batch growth kinetics of Mycobacterium bovis BCG

Maria Betania Batista Leal, Júlia Baruque-Ramos, Haroldo Hiss, Marcelo Fossa da Paz, Maria Cristina Sakai, Umbelina Macedo Vassoler, Luciana Juncioni de Arauz, Isaías Raw
2004 Brazilian Journal of Microbiology  
The influences of the L-asparagine and glycerol initial concentrations in Sauton medium on the productivities of biomass and colony forming units were studied. The submerged batch cultivations of Mycobacterium bovis were carried out in a 20 L bioreactor. The L-asparagine and glycerol initial concentrations of 4.54 g/L and 25 mL/L, respectively, corresponded to the best biomass productivity, namely 2.5 g/L.day. On the other hand, the concentrations of 2.27 g/L and 25 mL/L, respectively, led to
more » ... pectively, led to the highest productivity in terms of colony forming units, namely 2.7·10 6 colonies/mg.day. In addition, by means of the relative consumption analysis of L-asparagine and glycerol (50 and 26% respectively), it was concluded that the concentrations of such components could be reduced, with respect to the original Sauton medium composition, aiming the obtainment of an optimal BCG vaccine production in the bioreactor.
doi:10.1590/s1517-83822004000300013 fatcat:omy5bjnikbdh7att522gnpcdfu