Inhibition of Human Immunodeficiency Virus Type 1 Virion Entry by Dominant-Negative Hck

Kenzo Tokunaga, Etsuko Kiyokawa, Mie Nakaya, Naomi Otsuka, Asato Kojima, Takeshi Kurata, Michiyuki Matsuda
1998 Journal of Virology  
To study the role of Src family tyrosine kinases in infection with human immunodeficiency virus type 1 (HIV-1), we constructed an Hck mutant, HckN, that hinders signaling from wild-type Hck. HIV-1 produced in HckN-expressing cells was significantly less infectious to HeLa–CD4–LTR–β-gal (MAGI) cells than HIV-1 produced in mock-transfected cells. The inhibitory effect of HckN was compensated for by the expression of vesicular stomatitis virus G protein. Finally, we found that the HIV-1 produced
more » ... he HIV-1 produced in the HckN-expressing cells entered into the cells less efficiently than did the control HIV-1. These results suggest that the Src family tyrosine kinases regulate entry of HIV-1 into target cells.
doi:10.1128/jvi.72.7.6257-6259.1998 fatcat:63zzb4vjdvcz5glbvnntg56izi