The Effect of Different Single Doses from X-Rays on Pediatrics Rhabdomyosarcoma Tumor Cells: In Vitro Survival Curve

Alexander F.I. Osman
2016 Cancer therapy & Oncology International Journal  
the survival curve parameters for different normal and malignant cell organisms, including bone marrow stem cells, cartilage, epithelial cells, intestinal mucosa, parenchyma hepatocytes, hyperthermia, lung, skin colonies, skin fibroblast, Abstract The purpose of the work was to study the effect of different single radiation doses of X-rays on the pediatrics Rhabdomyosarcoma (RMS) cells in vitro. In addition, to construct the survival curve for the RMS cells and determine some crucial
more » ... crucial radiobiological parameters used for calculations of tumor cell kill in radiotherapy treatment of pediatrics RMS tumors. An established in vitro cell culture model employing the RMS tumor cell lines was used for this study. Cells were exposed to single doses of 0, 4, 8, 12, 16, and 20 Gy from a 6 MV X-rays photon beam energy. The clonogenic cell survival assay method was used to quantify the RMS cell survival. The survival curve was fitted by the single-hit multi-target and linear-quadratic models. The mean lethal dose (D 0 =3.2 Gy), the extrapolated number (n=11) represents a measure of the width of the survival curve shoulder and corresponds to the number of sub-lethal targets, and the dose at which the contributions of linear and quadratic components of cell killing are equally (α⁄β=9 Gy) were determined for the RMS cells. The survival curve parameters for the RMS cells were determined. Low radiosensitivity of cells under study in vitro was concluded. Conventional dose of 2 Gy per fraction should provide therapeutic gain with an increased tumor control probability (TCP) and decreased normal tissue complication probability (NTCP) for radiotherapy treatment.
doi:10.19080/ctoij.2016.01.555576 fatcat:qzgvp4v3qzg6tfqhv3ss6lnimu