Plant tissue culture provides a medium for the rapid growth and multiplication of economically important plants. It helps to develop mass amount of plantlets in a specific time and for bulk utilization. In the present study one medicinal plant, Eryngium foetidum, L was taken for the in vitro propagative study using various plant growth regulators. The shoot tips and leaf segments of Eryngium foetidum L were selected as explants for culture initiation. Different combination of selected auxin,

more »

... and cytokinin, BA were supplemented to the MS medium to assess the in vitro responses. High concentration of BA (6 mg/L) has produced best results by producing 6-8 shoot buds in shoot tip segments. Multiple shoots were produced at BA and BA with NAA. But BA above 8 mg/L reduce the shoot proliferation.