Direct versus indirect methods for growth measurements in batch cultures of Nitzschia palea

Ana Merse, Mireia Sánchez Humayor, Carolina Medina Alcaraz, Antera Martel Quintana, Juan Luis Gómez Pinchetti, Francesco Pisapia
2021 Zenodo  
Direct measurements such as cell density, are considered the most reliable methods for growth monitoring of microalgae. In comparison, indirect methods are undoubtedly less time-consuming, but their relevance needs to be evaluated case by case for the species and the experimental conditions used. Our study focused on the relevance of optical density (OD) and basal fluorescence (FO) measurements for batch cultures of the benthic diatom Nitzschia palea. Three Canarian strains of N. palea were
more » ... ured in triplicate 1 L Erlenmeyer flasks with 270 mL FDMed medium for 26 days with 1.3 x 105 cells mL-1 ± 27% (RSD). The three strains showed their maximum growth rate between days 4 and 7 (BEA0255B: 0.21 day-1 ± 0.023 (SD), BEA0256B: 0.23 day-1 ± 0.058 (SD), BEA0392B: 0.42 day-1 ± 0.065 (SD)), and entered the stationary phase at day 17, maintaining an average concentration of 1.5 x 106 cells mL-1± 11% (RSD). Comparison between cell density, OD and FO was performed using the data of each replicate of the three strains in the same analysis. The relevance of OD and FO measurements was demonstrated during the exponential phase, as they both positively correlated with cell density in a linear regression model (R2 > 0.89, n = 45). No linear correlation was found during the stationary phase (R2 < 0.46, n = 45). Further studies will address the study of photosynthetic efficiency, biomass weight and composition throughout the growth phases of N. palea, with the aim of determining the optimal harvesting time for biotechnology.
doi:10.5281/zenodo.7411767 fatcat:7g2bfati35ccvnki6j4vz5pcui