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Direct measurements such as cell density, are considered the most reliable methods for growth monitoring of microalgae. In comparison, indirect methods are undoubtedly less time-consuming, but their relevance needs to be evaluated case by case for the species and the experimental conditions used. Our study focused on the relevance of optical density (OD) and basal fluorescence (FO) measurements for batch cultures of the benthic diatom Nitzschia palea. Three Canarian strains of N. palea weredoi:10.5281/zenodo.7411767 fatcat:7g2bfati35ccvnki6j4vz5pcui