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House flies (Musca domestica L.) are one of the major pests found in poultry farms resulting in not only annoyance and indirect damage to the poultry production but also transmitting many infectious organisms mechanically and biologically. A highly pathogenic avian influenza virus H5N1 (HPAIV H5N1) collected from field house flies in Java Island, have fully succeeded identified and isolated in 2008. The AIV H5N1 isolates were further used in the present study to determine the presence anddoi:10.19087/jveteriner.2016.17.2.205 fatcat:2bpfblxkprf7pif7j2v6ecn7bu