A copy of this work was available on the public web and has been preserved in the Wayback Machine. The capture dates from 2018; you can also visit the original URL.
The file type is application/pdf
.
Cloning and Expression of an Oligo-1,6-glucosidase Gene from Arthrobacter globiformis I42 and Biochemical Characterization of the Recombinant Enzyme
2004
Journal of Applied Glycoscience
The gene encoding an oligo-1,6-glucosidase was cloned in terms of walking downstream from the glucodextranase gene of the chromosomal DNA of Arthrobacter globiformis I42. An open reading frame consisted of 1731 base pairs that encoded a mature protein of 577 amino acids (Mr, 63,000) was found. Transformed Escherichia coli cells carrying the 1.7-kb fragment overproduced the oligo-1,6-glucosidase under control of the T7 promoter of a pET system. Kinetic analyses of the recombinant protein gave Km
doi:10.5458/jag.51.37
fatcat:ai6xz6xx2jej5bljvaqypvxrpa