Phytogeographical origin of Madeiran common beans based on phaseolin patterns

Emanuel Marques da Silva, Anísia Soraia Abreu Correia, Nuno Alexandre Amaral Lopes, Humberto Gil Moreira Nóbrega, José Filipe Teixeira Ganança, Ana Maria Domingues, Manhaz Khadem, Jan Jacek Slaski, Miguel Ângelo Almeida Pinheiro de Carvalho
2010 Pesquisa Agropecuária Brasileira  
The objective of this work was to determine the geographic origin of the Madeiran common bean (Phaseolus vulgaris) gene pool. Phaseolin patterns of 50 accessions representing the diversity of common bean collected in Madeira, Portugal, and conserved in the ISOPlexis Germplasm Bank, were analysed using the Experion automated electrophoresis system, based on lab-on-a-chip technology. Five common bean standard varieties with typical phaseolin patterns were used to determine the phytogeographical
more » ... phytogeographical origin of the Madeiran common bean accessions. Ninety two percent of the accessions exhibited a phaseolin pattern consistent with the one of common bean types belonging to the Andean gene pool, while the origin of the remaining 8% of the accessions was indistinguishable. The application of a similarity coefficient of 85%, based on Pearson correlations, increases the number of accessions with uncertain pattern. The analytical approach used permitted the determination of the origin of the common bean gene pool, which is Andean in 98% of the cases, and clustering of the observed variability among the Madeiran common beans.
doi:10.1590/s0100-204x2010000800012 fatcat:7u37tzs2a5bnlnjoe3cotx27im