Design of simultaneous antimicrobial and anticoagulant surfaces based on nanoparticles and polysaccharides

Doris Breitwieser, Stefan Spirk, Hubert Fasl, Heike M. A. Ehmann, Angela Chemelli, Victoria E. Reichel, Christian Gspan, Karin Stana-Kleinschek, Volker Ribitsch
2013 Journal of materials chemistry. B  
The rational design of silver nanoparticles encapsulated in an anticoagulant, hemocompatible polysaccharide, 6-O-chitosan sulfate, is presented. Three different approaches are described for the immobilization of these core shell particles on cellulosic surfaces. The mass of the immobilized particles is quantified using a quartz crystal microbalance with dissipation (QCM-D). The antimicrobial activity of the surfaces towards E. coli MG 1655 [R1-16] is investigated by live/dead assays using
more » ... assays using fluorescence staining. All surfaces treated with the designed nanoparticles exhibit excellent antimicrobial activity towards E. coli MG 1655 [R1-16]. Anticoagulant properties of blood plasma on the nanoparticle treated surfaces have been determined using QCM-D. In comparison with the unmodified substrates, the total coagulation time as well as the thrombin formation time and fibrin clotting time of surfaces modified with nanoparticles are significantly increased.
doi:10.1039/c3tb00272a pmid:32260891 fatcat:o2gzw4z3lrhktiti6egl2qo56y