Toward multi-focal spot remote focusing two-photon microscopy for high speed imaging

Bei Li, Alexander D. Corbett, Ee Chong, Edward Mann, Tony Wilson, Martin J. Booth, Gil Bub, Thomas G. Brown, Carol J. Cogswell, Tony Wilson
2017 Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXIV  
Optical sectioning techniques using two-photon excitation of fluorescent indicators are central to diverse imaging applications. The limitations of the technique are low speed and undesirable specimen agitation. In our design, highspeed axial scanning is carried out by moving a reference objective to axially displace the focal spot without introducing significant spherical aberration and any agitation of the specimen. Further, the system is configured to allow switching between single spot and
more » ... ultiple focal spot remote focusing to allow either high dynamic range or high speed imaging. Downloaded From: on 03/15/2017 Terms of Use: Proc. of SPIE Vol. 10070 1007005-2 Downloaded From: on 03/15/2017 Terms of Use:
doi:10.1117/12.2251214 fatcat:gmen3z3o7jcdfjsinlkcqezwo4