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This paper addresses the optimization of continuous bioconversion process of glycerol to 1, by Klebsiella pneumoniae. The studied bioprocess is a complex nonlinear system that involves the gene regulation for dha regulon, enzymecatalytic kinetics on the reductive pathway, the active transport of glycerol and (passive) diffusion of 1,3-PD across the cell membrane, and the inhibition of glycerol dehydratase (GDHt) and 1,3-propanediol oxidoreductase (PDOR) by 3-hydroxypropionaldehy (3-HPA). Wedoi:10.7546/ijba.2018.22.3.213-228 fatcat:cgibonn6pbe33enupggpvb4dbm