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Measurement of in vivo expression of the recA gene of Escherichia coli by using lacZ gene fusions
1984
Journal of Bacteriology
A recA-lacZ protein fusion was constructed in vivo by using bacteriophage Mu d11301(Ap lac). The fusion contained the promoter and first 47 codons of the recA mutant, as determined by DNA sequence analysis. The fusion was cloned and used to construct a recA-lacZ operon fusion at the same site within the recA gene. These fusions were introduced into the Escherichia coli chromosome at the attachment site either as complete or cryptic prophages. Synthesis of P-galactosidase from these fusions was
doi:10.1128/jb.160.1.112-121.1984
fatcat:zbkaufnarzccrivfyctuhtv5b4