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Objective: To screen proteins of human pancreas cDNA library interacting with HCV NS3 protein. Methods: The library was amplifed, purified and evaluated, and then the puried library plasmids were transformed into yeast strain Y187. The reconstructed plasmid pGBKT7-NS3 was transformed into yeast strain AH109 and screened on the nutrient deficiency medium SD/-Trp. The transformed AH109 mated with Y187 containing the library plasmid. The diploid yeast cells were plated on nutrient deficiencydoi:10.1016/s1201-9712(09)60146-6 fatcat:wlmlfa4ugzbrnc446v2kwf7x5i