Effects of Crocus sativus L. (saffron) on the activity of CYP2A6, Xanthine Oxidase (XO) and N-acetyltransferase-2 (NAT2) in healthy volunteers [article]

Ευτυχία Ασπροδίνη, Μαρία Δαβίτη, University Of Thessaly, University Of Thessaly
Θέμα: "Μελέτη της επίδρασης του Crocus sativus L. (σαφράν) στη λειτουργικότητα των ενζύμων CYP2A6, Οξειδάση της Ξανθίνης (ΧΟ) και Ν-ακετυλοτρανσφεράσης-2 (ΝΑΤ2) σε υγιείς εθελοντές" Μαρία Δαβίτη Τριμελής εξεταστική επιτροπή: • Ευτυχία Ασπροδίνη, Αναπληρώτρια Καθηγήτρια (Επιβλέπουσα) • Αναστάσιος Γερμενής, Καθηγητής Ανοσολογίας  Ανδρέας Τσακάλωφ, Αναπληρωτής Καθηγητής Χημείας Διπλωματική Εργασία υποβληθείσα στο Τμήμα Ιατρικής του Πανεπιστημίου Θεσσαλίας ως μέρους των απαιτήσεων για την απόκτηση
more » ... ων για την απόκτηση Μεταπτυχιακού Διπλώματος Ειδίκευσης στη Φαρμακολογία Λάρισα, Οκτώβριος, 2018 ΠΑΝΕΠΙΣΤΗΜΙΟ ΘΕΣΣΑΛΙΑΣ ΣΧΟΛΗ ΕΠΙΣΤΗΜΩΝ ΥΓΕΙΑΣ Τμήμα ΙΑΤΡΙΚΗΣ Abstract Introduction: Saffron is the dried stigmas of Crocus sativus L. and it is one of the most expensive spices in terms of price by weight. The dried stigmas are widely used in foods as a flavoring and coloring agent, while it is also used as herbal medicine due to its medicinal properties. The main components of saffron are picrocrocin, safranal, crocin and crocetin. The aim of this study was to investigate the in vivo effect of saffron tea on the enzymatic activities of CYP2A6, XO and NAT2, as there is complete lack of in vivo evidence of its effects in humans. Methods: The sample consisted of 9 healthy volunteers. They followed a special diet during which they abstained from carotenoid-containing foods for 14 days, while they consumed the saffron tea (300mg / 150ml) on a daily basis for the last 7 days. Caffeine was used as a metabolic probe by estimating the urinary metabolic ratios in volunteers. In total, 54 urine samples were obtained that were collected in three different stages of the protocol, phase I: before the onset of the carotenoid-free diet, phase II: before saffron's administration and phase III: after saffron's administration. At each phase of the protocol, two urine samples were obtained: the first sample consisted of urine collected the first five hours after caffeine consumption (0-5h) and the second sample consisted of urine collected at the sixth hour (6h). Results: The mean values of caffeine metabolic ratios for CYP2A6, XO and NAT2 enzymes in the urine samples 0-5h did not show a statistically significant change. Similarly, the mean values of caffeine metabolic ratios for CYP2A6, XO and NAT enzymes in the 6h samples showed no statistically significant change. Conclusion: Regular administration of the saffron tea at a dose of 300mg / 100ml per day for 7 days does not appear to affect the activity of the enzymes CYP2A6, XO and NAT2. Therefore, regular saffron tea may be considered safe either when it is consumed alone, or in combination of drugs that are substrates of the above-mentioned enzymes.
doi:10.26253/heal.uth.6760 fatcat:icx4uuahavdlppzyfzlryibyse